Myosin forces remodel F-actin for mechanosensitive protein recognition
Unless otherwise noted, chemicals and buffer components were purchased from Sigma-Aldrich. Cell culture PtK2 cells (ATCC, CCL-56) were cultured in MEM (Gibco) supplemented with 10% fetal bovine serum (FBS), 1 mM sodium pyruvate, 1× non-essential amino acids (Gibco), and 1× antibiotic–antimycotic (Gibco) at 37 °C in 5% CO2. All cell lines were obtained from ATCC, which validates…