Structural basis of fungal β-1,3-glucan synthase inhibition by caspofungin
ScFks1 expression and purification ScFks1 was expressed and purified from S. cerevisiae BY4741 fks2 deletion strain18 harbouring the genomic copy of the fks1 gene engineered to have a C-terminal HRV3C protease site, ten amino acid linker, 3× Flag tag, and 10× His-tag. The fks1 gene was modified by homologous recombination by following a standard procedure42. To…