Cloning, protein expression and purification The DNA encoding ThermoCas9 with a C-terminal His6 tag was integrated into the pML-1B vector and expressed in the Escherichia coli NiCo21(DE3) strain. Cells were grown in Luria–Bertani (LB) medium with 0.2% d-(+)-glucose at 37 °C until optical density at 600 nm reached 0.8, at which point addition, isopropyl-β-d-thiogalactopyranoside was added to 0.5 mM…
DeChambeau’s LIV contract is up at the end of this season and he was looking to sign a lucrative new deal before Saudi Arabia’s Public Investment Fund (PIF) announced it was set to pull its financial support, with reports, external suggesting he wants $500m (£370m) to stay. The two-time US Open winner said he was…
Kofi Kingston and Xavier Woods left WWE in a surprising mutual departure on May 2, 2026, after rejecting a TKO contract restructure. JC Mateo and Tonga Loa also exited. Source link
The Terminally Ill Adults (End of Life) Bill was supported by MPs but has not cleared its stages in the Lords. Source link
At a training course at a Shoreditch police station the following year, Manning was discussing “difficult situations” with fellow officers and decided to show them the photo of the dead man, saying: “I’ve been to a bad one, I will show you the picture.” Source link
Academy Award-winning actress and producer Octavia Spencer will co-host a virtual fundraising event with Hillary Clinton and Nancy Pelosi on June 1, 2026. Clinton made the announcement on her social media account this week. Clinton’s announcement put it plainly: “Academy-Award winning actress and producer Octavia Spencer will join me and @TeamPelosi for our June 1…
Ether (ETH) futures on Binance have risen to a near two-month high as aggressive buyers stepped into the market over the past week. Buy-taker volume rose above $5 billion, and the current setup suggests the ETH rally is poised to continue. On Binance, the 24-hour cumulative net taker volume reached $5.5 billion, rising 72% from…
Prime Minister Sanae Takaichi is attempting to raise Japan’s profile at a time when China’s clout is rising and doubts about the United States are spreading. Source link
In an era when digital collaboration and online resources are critical to the distribution and advancement of academic and scientific knowledge, the importance of internet safety in these fields has grown dramatically. The transition to online publishing, virtual conferences, and web-based collaboration has exposed the academic and scientific communities to a slew of cyber threats….
The delay is another hurdle in the Trump administration’s push to secure an agreement that would curb Iran’s nuclear program. Source link