Synthetic super-enhancers enable precision viral immunotherapy

Destination vector cloning We built a specific custom destination vector for efficient Golden Gate cloning of enhancer fragments. This included the reporter gene cassette NanoLuc-Ires-mNGreen-pA, downstream of a mCMV promoter. A bacterial suicide ccdB cassette spanning the enhancer position enabled assembly of a single enhancer for selection and efficient cloning. This was based on the…

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Challenge Cup quarter-final: Benetton 41, Exeter Chiefs 44

Benetton: Gallagher; Ratave, Menoncello, Fekitoa, Mendy; Umaga, Uren; Gallo, Bernasconi, Zilocchi, N Cannone, Ruzza, Zuliani, Favretto, L Cannone Replacements: Maile, Aminu, Pasquali, Negri, Fa’aso’o, Izekor, A Garbisi, Marin Exeter Chiefs: Woodburn; Feyi-Waboso, Slade, Rigg, Ridl; Skinner, Varney; Sio, Yeandle, Roots, Jenkins, Zambonin, Hooper, Vintcent, Fisilau Replacements: Dweba, Burger, Tchumbadze, Tuima, Worley-Brady, Cairns, Haydon-Wood, John Referee:…

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