Ubiquitination of glycogen and metabolites in cells and tissues

Ubiquitin signalling covers a vast realm of protein modifications, yet may still be underestimated due to non-proteinaceous substrates, such as sugars, lipids, and nucleotides1 . The breadth of ubiquitinated non-protein substrates, their abundance, and cellular roles are currently unclear, since current ubiquitinomic and proteomic techniques are blind to non-proteinaceous modifications. We report Non-Protein Ub-clipping (NoPro-clipping) as…

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Structural basis of fungal β-1,3-glucan synthase inhibition by caspofungin

ScFks1 expression and purification ScFks1 was expressed and purified from S. cerevisiae BY4741 fks2 deletion strain18 harbouring the genomic copy of the fks1 gene engineered to have a C-terminal HRV3C protease site, ten amino acid linker, 3× Flag tag, and 10× His-tag. The fks1 gene was modified by homologous recombination by following a standard procedure42. To…

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