HIV-1 signalling remodels nuclear pores to licence infection

Cells Peripheral blood mononuclear cells (PBMCs) were isolated from leukocyte cones from healthy donors (NHS Blood and Transplant Services) by density centrifugation using Lympho 24+ Spin medium (PluriSelect) and pluriMate II tubes (PluriSelect). PBMCs were cryopreserved in 10% DMSO (Sigma-Aldrich) in FCS (LabTech). CD4+ T cells were isolated by negative selection using the MojoSort Human…

Read More

Multiplexed magnetic resonance imaging | Nature

Lauterbur, P. C. Image formation by induced local interactions: examples employing nuclear magnetic resonance. Nature 242, 190–191 (1973). Article  ADS  CAS  Google Scholar  Mansfield, P. Multi-planar image formation using NMR spin echoes. J. Phys. C Solid State Phys. 10, L55–L58 (1977). Article  ADS  CAS  Google Scholar  Wattjes, M. P. et al. Evidence-based guidelines: MAGNIMS consensus…

Read More

Purcell-enhanced spin–phonon coupling with a single colour centre

Cohen-Tannoudji, C., Dupont-Roc, J. & Grynberg, G. Atom-Photon Interactions: Basic Processes and Applications (Wiley, 1998). Purcell, E. M. Spontaneous emission probabilities at radio frequencies. Phys. Rev. 69, 681 (1946). Google Scholar  Walther, H., Varcoe, B. T. H., Englert, B.-G. & Becker, T. Cavity quantum electrodynamics. Rep. Prog. Phys. 69, 1325–1382 (2006). ADS  Google Scholar  Blais,…

Read More

Steric hindrance of antibody binding in an Omicron spike fusion intermediate

Low, J. S. et al. ACE2-binding exposes the SARS-CoV-2 fusion peptide to broadly neutralizing coronavirus antibodies. Science 377, 735–741 (2022). Article  ADS  CAS  PubMed  PubMed Central  Google Scholar  Sun, X. et al. Neutralization mechanism of a human antibody with pan-coronavirus reactivity including SARS-CoV-2. Nat. Microbiol. 7, 1063–1074 (2022). Article  CAS  PubMed  Google Scholar  Qu, P….

Read More

Prefrontal to ventral tegmental area dynamics drive contingency degradation

Kesner, R. P. & Churchwell, J. C. An analysis of rat prefrontal cortex in mediating executive function. Neurobiol. Learn. Mem. 96, 417–431 (2011). PubMed  Google Scholar  Dalley, J. W., Cardinal, R. N. & Robbins, T. W. Prefrontal executive and cognitive functions in rodents: neural and neurochemical substrates. Neurosci. Biobehav. Rev. 28, 771–784 (2004). CAS  PubMed …

Read More

Tree community resource economics control soil food web multifunctionality

Study sites and sampling design We used a pan-European network of 64 mature, uneven-aged forest plots (30 × 30 m2) consisting of three-species mixture stands (34 plots) and corresponding monospecific stands (30 plots; Extended Data Table 1). These plots are part of the FunDivEUROPE (Functional Significance of Forest Biodiversity in Europe) exploratory platform59, and were established across European forests…

Read More